Madrid were handed a shock 2-1 defeat at the hands of newly-promoted Girona leaving them eight points off league leaders Barcelona after 10 games.
And tempers boiled over during the loss at Girona's Montilivi ground when Marcelo lost possession, leading team-mate Modric to berate the Brazilian.
Marcelo was caught gesturing and shouting at his team-mate Luka Modric during the game
Modric criticised Marcelo after the left back lost the ball during the shock loss on Sunday
Spanish outlet AS report that Marcelo took exception to Modric's criticism and responded angrily: 'You say that I've messed up… and you haven't!'.
The pair eventually made up but they weren't the only Madrid players showing signs of frustration as Los Blancos lost in their first ever competitive match against Girona.
Isco gestured angrily towards Karim Benzema while Cristiano Ronaldo was annoyed at centre back pairing Raphael Varane and Sergio Ramos for positioning themselves in his path as he went to attack a set piece.
Madrid took an early lead through Isco but Girona turned the game on it's head with two second-half goals in quick succession, as Cristhian Stuani and Portu scored in the 54th and 58th minute to stun the Spanish champions.
Modric and Marcelo embrace during Real Madrid's 3-0 home win over Eibar earlier this month
Zinedine Zidane remained calm despite Madrid's worst start to a season since 2012-13
Zinedine Zidane was calm after the match insisting there was still a long way to go in the title race and pointed to a lack of concentration in key moments as the reason for the loss to Girona.
The result means Madrid have made their worst start to a league season since the 2012-13 campaign when they finished 15 points off champions Barcelona.
Madrid must now turn their attentions to Europe ahead of Wednesday night's match against Tottenham at Wembley in the Champions League group stages.
The holders drew 1-1 with Spurs at the Bernabeu in their first meeting earlier this month with Ronaldo's penalty cancelling out a Varane own goal.